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	Provedor de dados ArchiMer (5) Autor Avarre, Jean-christophe (5) Alonso, Pascal (1) Ansquer, Dominique (1) Bayu Intan, Mariana D. (1) Boulo, Viviane (1) Cochet, C. (1) De Lorgeril, Julien (1) Diombokho, A. (1) Dugue, R. (1) Durand, Jean Dominique (1) Escoubas, Jean-michel (1) Faivre, N. (1) Iwataki, Mitsunori (1) Joffrois, C. (1) Le Moullac, Gilles (1) Levy, Peva (1) Masseret, Estelle (1) Montagnani, Caroline (1) Muawanah, (1) Quiquand, M (1) Mais... Palavra-chave Capacité osmorégulatrice (1) Crassostrea gigas (1) Damage associated molecular pattern (1) Diagnostic (1) Dinoflagellate (1) Environnement (1) Gene expression (1) Harmful algal blooms (1) Indonesia (1) Margalefidinium polykrikoides (1) Metalloproteinase (1) Mollusk immunity (1) Non-model organism (1) Nouvelle-Calédonie (1) Oyster (1) PCR (1) PCR diagnosis (1) Penaeus (Litopenaeus) stylirostris (1) Reference gene (1) Resting and hyaline cysts (1) Mais... Tipo do documento Text (5) Ano 2019 (1) 2014 (1) 2007 (1) 2000 (1) 1998 (1) País France (5) Idioma Inglês (4) Francês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Analysis of the black-chinned tilapia Sarotherodon melanotheron heudelotii reproducing under a wide range of salinities: from RNA-seq to candidate genes ArchiMer Avarre, Jean-christophe; Dugue, R.; Alonso, Pascal; Diombokho, A.; Joffrois, C.; Faivre, N.; Cochet, C.; Durand, Jean Dominique. The black-chinned tilapia Sarotherodon melanotheron heudelotii is an ecologically appealing model as it shows exceptional adaptive capacities, especially with regard to salinity. In spite of this, this species is devoid of genomic resources, which impedes the understanding of such remarkable features. De novo assembly of transcript sequences produced by next-generation sequencing technologies offers a rapid approach to obtain expressed gene sequences for non-model organisms. It also facilitates the development of quantitative real-time PCR (qPCR) assays for analysing gene expression under different environmental conditions. Nevertheless, obtaining accurate and reliable qPCR results from such data requires a number of validations prior to interpretation.... Tipo: Text Palavras-chave: Gene expression; Non-model organism; Reference gene; Salinity; Spermatogenesis; Tilapia. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28255/26625.pdf First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway ArchiMer Montagnani, Caroline; Avarre, Jean-christophe; De Lorgeril, Julien; Quiquand, M; Boulo, Viviane; Escoubas, Jean-michel. In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and... Tipo: Text Palavras-chave: Damage associated molecular pattern; Tissue inhibitor of metalloproteinase; Metalloproteinase; Mollusk immunity; Crassostrea gigas; Oyster. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2094.pdf Rapid and sensitive PCR detection of Vibrio penaeicida, the putative etiological agent of syndrome 93 in New Caledonia ArchiMer Saulnier, Denis; Avarre, Jean-christophe; Le Moullac, Gilles; Ansquer, Dominique; Levy, Peva; Vonau, Vincent. Experimental infections of Penaeus (Litopenaeus) stylirostris were performed with a Vibrio penaeicida strain (AM101) isolated in New Caledonia from Syndrome 93 diseased shrimp. Cumulative mortalities resulting from intramuscular injection or immersion of shrimp in bacterial suspensions demonstrated high virulence for this bacterial strain and suggested that V. penaeicida could be the etiological agent of Syndrome 93. The median lethal dose (LD50) for AM101 was 1.3 x 104 CFU (colony forming units) ml-1 by immersion and less than 5 CFU shrimp-1 by intramuscular challenge, with mortality outbreaks at 48 and 22 h after challenge, respectively. A polymerase chain reaction (PCR) detection assay using a primer set designed from the 16S ribosomal RNA gene of V.... Tipo: Text Palavras-chave: Small subunit rDNA gene; Penaeus (Litopenaeus) stylirostris; Vibriosis; Shrimp disease; PCR diagnosis; Vibrio penaeicida; Syndrome 93. Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-4436.pdf Rapport de la mission effectuée du 12 au 19/12/97en Nouvelle-Calédonie : - Ecophysiologie et Pathologie ArchiMer Soyez, Claude; Avarre, Jean-christophe. Ecophysiologie L'outil Capacité Osmorégulatrice (CO) mis au point ces dernières années est devenue un moyen performant et largement exploité en routine dans nos programmes de recherche en environnement, en pathologie-immunologie, en génétique et en physiologie de la nutrition. La mise en oeuvre de cet outil nous a permis de répondre à un certain nombre de questions posées dans le cadre de nos objectifs de recherche. Il s'agissait dans un deuxième temps d'éprouver et d'adapter cet outil à des études in situ (dans les bassins d'élevages). C'est dans ce cadre qu'une action a été menée en Nouvelle-Calédonie avec nos collègues de la SASV. En Nouvelle-Calédonie, la chute de température préside à une dégradation du milieu et des conditions d'élevage et... Tipo: Text Palavras-chave: Capacité osmorégulatrice; Stress; Environnement; Écophysiologie; Nouvelle-Calédonie; Diagnostic; V. penaeicida; Épidémiologie; PCR. Ano: 1998 URL: https://archimer.ifremer.fr/doc/00680/79233/81748.pdf Resting Cyst Distribution and Molecular Identification of the Harmful Dinoflagellate Margalefidinium polykrikoides (Gymnodiniales, Dinophyceae) in Lampung Bay, Sumatra, Indonesia ArchiMer Thoha, Hikmah; Muawanah,; Bayu Intan, Mariana D.; Rachman, Arief; Sianturi, Oksto Ridho; Sidabutar, Tumpak; Iwataki, Mitsunori; Takahashi, Kazuya; Avarre, Jean-christophe; Masseret, Estelle. Margalefidinium polykrikoides, an unarmored dinoflagellate, was suspected to be the causative agent of the harmful algal blooms – associated with massive fish mortalities – that have occurred continually in Lampung Bay, Indonesia, since the first bloom event in October 2012. In this study, after examination of the morphology of putative M. polykrikoides-like cysts sampled in bottom sediments, cyst bed distribution of this harmful species was explored in the inner bay. Sediment samples showed that resting cysts, including several morphotypes previously reported as M. polykrikoides, were most abundant on the northern coast of Lampung Bay, ranging from 20.6 to 645.6 cysts g-1 dry sediment. Molecular phylogeny inferred from LSU rDNA revealed that the so-called... Tipo: Text Palavras-chave: Margalefidinium polykrikoides; Dinoflagellate; Harmful algal blooms; Resting and hyaline cysts; Indonesia. Ano: 2019 URL: https://archimer.ifremer.fr/doc/00489/60086/63423.pdf Registros recuperados: 5 Primeira ... 1 ... Última

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